Endoplasmic Reticulum

Cell Biology

Endoplasmic Reticulum Definition

The endoplasmic reticulum (ER) is a large organelle made of membranous sheets and tubules that begin near the nucleus and extend across the cell. The entire structure can account for a large proportion of the endomembrane system of the cell. For instance, in cells such as liver hepatocytes that are specialized for protein secretion and detoxification, the ER can account for more than 50% of the total lipid bilayer of the cell. Similarly, the ER membrane system is particularly prominent in pancreatic beta cells that secrete insulin, or within activated B-lymphocytes that produce antibodies.

Endomembrane system diagram

As seen in the image, the membranes of the endoplasmic reticulum are contiguous with the outer nuclear membrane, even though their compositions can be different. The ER contains special membrane-embedded proteins that stabilize its structure and curvature. This organelle acts as an important regulator of cell function because it interacts closely with a number of other organelles – from the plasma membrane and Golgi network, to mitochondria and peroxisomes. It can even influence mitochondrial fission, and change the aerobic status of the cell.

Clara cell lung - TEM

This is a microscopic image of a section from mammalian lung tissue. The bottom right corner of the image shows the nucleus and the rest of the picture illustrates the extensive nature of the ER. Small dark circles are mitochondria that exist in physical proximity with the membranes of the ER.

Types of Endoplasmic Reticulum

There are two major types of ER within each cell – smooth endoplasmic reticulum (SER) and rough endoplasmic reticulum (RER). Each has distinct functions, and often, differing morphology. The SER is involved in lipid metabolism and acts as the calcium store for the cell. This is particularly important in muscle cells that need Ca2+ ions for contraction. The SER is also involved in the synthesis of phospholipids and cholesterol. It is often located near the periphery of the cell.

On the other hand, the RER is commonly seen close to the nucleus. It contains membrane-bound ribosomes that give it the characteristic ‘rough’ appearance. These ribosomes are creating proteins that are destined for the lumen of the ER and are moved into the organelle as they are being translated. These proteins contain a short signal created by a few amino acids in their N-terminus, and are initially translated in the cytoplasm. However, as soon as the signal is translated, special proteins bind to the growing polypeptide chain and move the entire ribosome and associated translation machinery to the ER. These polypeptides could be resident proteins of the RER, or be moved towards the Golgi network to be sorted and secreted.

Structure of the Endoplasmic Reticulum

The endoplasmic reticulum membrane system can be morphologically divided into two structures–cisternae and sheets. Cisternae are tubular in structure, and form a three-dimensional polygonal network. They are about 50 nm in diameter in mammals and 30 nm in diameter in yeast. ER sheets, on the other hand, are membrane-enclosed, two-dimensional flattened sacs that extend across the cytoplasm. They are frequently associated with ribosomes and special proteins called translocons that are necessary for protein translation within the RER.

Endoplasmic Reticulum Structure

The high-curvature of ER tubules is stabilized by the presence of proteins called reticulons and DP1/Yop1p. Reticulons are membrane-associated proteins encoded by four genes in mammals (RTN1-4). These proteins localize to ER tubules and the curved edges of ER sheets. DP1/Yop1p are a class of integral membrane proteins involved in stabilizing the structure of ER cisternae. Both reticulons and DP1/Yop1 proteins form oligomers and interact with the cytoskeleton. Oligomerization seems to be one of the mechanisms used by these proteins to shape the lipid bilayer into a tubule. Additionally, they also appear to use a wedge-like structural motif that causes the membrane to curve. These two classes of proteins are redundant, since the overexpression of one protein can compensate for the lack of the other protein.

The construction of the ER is intimately involved with the presence of cytoskeletal elements – especially microtubules. ER membranes, especially cisternae, move and branch along microtubules. When microtubule structure is temporarily disrupted, the ER network collapses and reforms only after the microtubule cytoskeleton is reestablished. In addition, changes to the pattern of microtubule polymerization are reflected in changes to ER morphology.

Functions of Endoplasmic Reticulum

The ER plays a number of roles within the cell, from protein synthesis and lipid metabolism to detoxification of the cell. Cisternae are commonly associated with lipid metabolism since they often create SER. They also appear to be important in maintaining the Ca2+ balance within the cell and in the interaction of the ER with mitochondria. This interaction also influences the aerobic status of the cell.

ER sheets appear to be crucial in the response of the organelle to stress, especially since cells alter their tubules:sheets ratio when the number of unfolded proteins increases. Occasionally, apoptosis is induced by the ER in response to an excess of unfolded protein within the cell. When ribosomes detach from ER sheets, these structures can disperse and form tubular cisternae.

Although ER sheets and tubules appear to have distinct functions, there isn’t a perfect delineation of roles. For instance, in mammals, tubules and sheets can interconvert. In yeast, ribosomes are found on ER tubules and mammalian ER cisternae can contain translocon proteins as well. The relationship between structure and function in the ER has not been completely elucidated.

Protein Synthesis and Folding

Protein synthesis occurs in the RER. Although translation for all proteins begins in the cytoplasm, some are moved into the ER in order to be folded, and sorted for different destinations. Proteins that are translocated into the ER during translation are often destined for secretion. Initially, these proteins are folded within the ER and then moved into the Golgi apparatus where they can be dispatched towards other organelles. For instance, the hydrolytic enzymes in the lysosome are generated in this manner. Alternately, these proteins could be secreted from the cell. This is the origin of the enzymes of the digestive tract. The third potential role for proteins translated in the ER is to remain within the endomembrane system itself. This is particularly true for chaperone proteins that assist in the folding of other proteins. The genes encoding these proteins are upregulated when the cell is under stress from unfolded proteins.

Lipid Synthesis

The SER plays an important role in cholesterol and phospholipid biosynthesis. Therefore, this section of the ER is important not only for the generation and maintenance of the plasma membrane, but of the extensive endomembrane system of the ER itself. The SER is enriched in enzymes involved in sterol and steroid biosynthetic pathways and is also necessary for the synthesis of steroid hormones. Therefore the SER is extremely prominent in the cells of the adrenal gland that secrete five different groups of steroid hormones that influence the metabolism of the entire body. The synthesis of these hormones also involves enzymes within the mitochondria, further underscoring the relationship between these two organelles.

Calcium Store

The SER is an important site for the storage and release of calcium in the cell. A modified form of the SER called sarcoplasmic reticulum forms an extensive network in contractile cells such as muscle fibers. Calcium ions are also involved in the regulation of metabolism in the cell, and can change cytoskeletal dynamics. The extensive nature of the ER network allows it to interact with the plasma membrane and use Ca2+ for signal transduction and modulation of nuclear activity. In association with mitochondria, the ER can also use its calcium stores to induce apoptosis in response to stress.

Related Biology Terms

  • Apoptosis – Programmed cell death that occurs in multicellular organisms, preceded by distinct changes to the morphology and biochemistry of the cell. Common during development and also used to prevent disease.
  • Endosymbionts – Organisms that live within other organisms. Mitochondria are considered ancient endosymbionts of early eukaryotic cells.
  • Motor Proteins – Proteins that function as molecular motors, converting chemical energy to mechanical energy, while moving along a suitable surface.
  • Upregulation – In genetics, refers to an increase in the number of RNA transcripts produced off a gene. Can also refer to the increase in the number of receptors found on a cell surface.


1. Which of these is true about the endoplasmic reticulum?
A. Dependent on the cytoskeleton
B. Made of tubular cisternae that are 100 nm in diameter
C. Is contiguous with the plasma membrane and therefore ideal for protein secretion
D. All of the above

Answer to Question #1

2. Which of these is a function of smooth endoplasmic reticulum?
A. Synthesis and secretion of steroid hormones
B. Maintenance and regeneration of the plasma membrane
C. Storage and release of Ca2+ ions
D. All of the above

Answer to Question #2

3. Which of these is true about the functions of proteins that stabilize the structure of the endoplasmic reticulum?
A. Translocons are important in maintaining the curvature of cisternae
B. DP1/Yop1p group of proteins can create oligomers that form a scaffold and stabilize the curved regions of the ER
C. Reticulons are capable of forming wedge-like structures that insert themselves into the plasma membrane and anchor the ER
D. All of the above

Answer to Question #3

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